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Single Cell & Spatial Transcriptomics Data Analysis

Digging Transcriptomics Data at Single Cell Level

Single-cell and spatial transcriptomics (RNA-seq) has revolutionized our ability to generate hypothesis-driven, publication-quality data by providing unprecedented insights into cellular heterogeneity and gene expression patterns at the individual cell level. This powerful technique allows researchers to explore complex biological systems with remarkable resolution, uncovering novel cell types, states, and regulatory mechanisms. We help our clients to generate scientifically meaningful data, for publication, grants, and fellowship applications. Please look at some example figures, to understand the quality and level of our work.

Expression level of GFAP

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Figure 1. Cell-type specific expression level of GFAP in Alzheimer's disease (AD) mouse brain. Representative box plot showing the expression level of GFAP in different cell types in AD mouse brain.

 

Source of raw data: Zeng H, Huang J, Zhou H, Meilandt WJ, Dejanovic B, Zhou Y, Bohlen CJ, Lee SH, Ren J, Liu A, Tang Z. Integrative in situ mapping of single-cell transcriptional states and tissue histopathology in a mouse model of Alzheimer’s disease. Nature Neuroscience. 2023 Mar;26(3):430-46.

Cell types

A

Control mouse

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B

Alzheimer's mouse

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Figure 2. Spatial distribution of GFAP in brain tissue of control and AD mouse. Representative tissue level spatial expression pattern of GFAP in (A) control and (B) AD mouse brain.

 

Source of raw data: Zeng H, Huang J, Zhou H, Meilandt WJ, Dejanovic B, Zhou Y, Bohlen CJ, Lee SH, Ren J, Liu A, Tang Z. Integrative in situ mapping of single-cell transcriptional states and tissue histopathology in a mouse model of Alzheimer’s disease. Nature Neuroscience. 2023 Mar;26(3):430-46.

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